Table 1. List of primers used for real-time polymerase chain reaction

Primers		Sequences 5’→ 3’	Annealing temperature	Reference genes (GenBank no.)
qPCR	GAPDH-F	5’ - GAG GGT AGT GAA GGC TGC TG - 3’	58.0°C	XM_015873412.2
	GAPDH-R	5’ - ACC AGG AAA CAA GCT TGA CG - 3’
	PAX3-F	5’ - AGC AAC TGG AAG AGC TGG AAA G -3’	64.0°C	XM_015871337.2
	PAX3-R	5’ - CTC CTG GGA TCA GGT GGT TAA A - 3’
	PAX7-F	5’ - AGC TGG CAG AGA TGG AGT TG - 3’	64.0°C	XM_032448793.1
	PAX7-R	5’ - CTA GTG GTG GTG GTG GCA AA - 3’
	MYF5-F	5’ - AGG AGG CTG AAG AAA GTG AAC C -3’	60.0°C	XM_015857363.2
	MYF5-R	5’ - TAG TTC TTC ACC TGT TCC CTC A - 3’
	MYOD1-F	5’ - CAG CTA CTA CAC AGA ATC ACC AA - 3’	63.2°C	XM_015864142.1
	MYOD1-R	5’ - TCC CTT CAG CAA CAG CTT CA - 3’
	MYOG-F	5’ - TGC CCA AGG TGG AGA TCC TA - 3’	63.2°C	XM_015884883.2
	MYOG-R	5’ - GGG TTG GTG CCA AAC TCC AG - 3’
	FERMT2-F	5’ - CCA TAC TGG TGC ACG TTC A - 3’	60.0°C	XM_015865947.2
	FERMT2-R	5’ - CAG CCA GAT TTC ATT CAT TCC C - 3’
	DES-F	5’ - TCT CAA TTT CCG AGA GAC CAG - 3’	56.2°C	XM_015869160.2
	DES-R	5’ - CCA CCT CTC CAT CAC GAG TT - 3’
	CAV3-F	5’ - GCT TTG ATG GTG TCT GGA AA - 3’	53.4°C	XM_015875457.2
	CAV3-R	5’ - ATG TGG CAG AAG GAA ATG AG - 3’