Table 6. Effect of dietary Bacillus subtilis and oregano essential oil supplementation on intestinal morphology(H&E stain) of late-phase laying hens

Villi height (μm) Crypt depth (μm) Villi height / Crypt depth
Egg production
H 592.80 103.13a 5.85b
A 610.76 99.06b 6.22a
SEM 8.501 1.402 0.078
Additive
CON 584.23b 111.55a 5.27b
BS 622.20a 98.59b 6.37a
OEO 598.89ab 93.15c 6.45a
SEM 10.412 1.717 0.096
Egg production×Additive
H CON 587.90bc 117.87a 5.00c
BS 631.38a 101.53bc 6.28a
OEO 559.11c 89.99d 6.26a
A CON 580.57bc 105.23b 5.54b
BS 613.02ab 95.64cd 6.47a
OEO 638.67a 96.32cd 6.64a
SEM
P-values
Egg production 0.1375 0.042 0.0011
Additive 0.0368 <.0001 <.0001
Interaction 0.0018 0.0006 0.4553
Means in same rows with different superscripts are significantly different (P<0.05).
H, high egg production; A, average egg production; CON, fed a basal diet; BS, fed basal diet plus 3 × 108 CFU/kg feed Bacillus subtilis; OEO, fed basal diet plus 0.3 g/kg feed oregano essential oil.