Table 6. Effect of dietary Bacillus subtilis and oregano essential oil supplementation on intestinal morphology(H&E stain) of late-phase laying hens
| Villi height (μm) | Crypt depth (μm) | Villi height / Crypt depth |
Egg production |
H | 592.80 | 103.13a | 5.85b |
A | 610.76 | 99.06b | 6.22a |
SEM | 8.501 | 1.402 | 0.078 |
Additive |
CON | 584.23b | 111.55a | 5.27b |
BS | 622.20a | 98.59b | 6.37a |
OEO | 598.89ab | 93.15c | 6.45a |
SEM | 10.412 | 1.717 | 0.096 |
Egg production×Additive |
H | CON | 587.90bc | 117.87a | 5.00c |
BS | 631.38a | 101.53bc | 6.28a |
OEO | 559.11c | 89.99d | 6.26a |
A | CON | 580.57bc | 105.23b | 5.54b |
BS | 613.02ab | 95.64cd | 6.47a |
OEO | 638.67a | 96.32cd | 6.64a |
SEM |
P-values |
Egg production | 0.1375 | 0.042 | 0.0011 |
Additive | 0.0368 | <.0001 | <.0001 |
Interaction | 0.0018 | 0.0006 | 0.4553 |
Means in same rows with different superscripts are significantly different (P<0.05).
H, high egg production; A, average egg production; CON, fed a basal diet; BS, fed basal diet plus 3 × 108 CFU/kg feed Bacillus subtilis; OEO, fed basal diet plus 0.3 g/kg feed oregano essential oil.