Table 4. Effect of dietary Bacillus subtilis and oregano essential oil supplementation in serum biochemical profiles of late-phase laying hens

		TC	TG	GLU	TP	AST	ALT	Ca	Crea	ALB	LDH
Egg production
H		170.28	1,573.20	174.19	6.44	224.78	0.45	29.41	0.32	2.17	1,392.30
A		159.75	1,415.66	178.73	6.47	231.07	0.62	28.32	0.32	2.19	1,563.83
SEM		7.697	39.230	5.859	0.089	14.898	0.106	0.429	0.004	0.021	101.246
Additive
CON		177.38	1,516.26	170.54	6.41	215.81	0.48	28.96	0.33	2.19	1,454.51
BS		151.44	1,532.10	170.16	6.41	242.44	0.65	28.52	0.32	2.16	1,591.60
OEO		166.23	1,434.93	188.67	6.56	225.53	0.47	29.11	0.32	2.19	1,388.09
SEM		9.427	48.047	7.175	0.109	18.246	0.130	0.526	0.005	0.026	124.000
Egg production×Additive
H	CON	169.76	1,595.20	163.79	6.32	219.18	0.50	29.17	0.33	2.18	1,331.9
	BS	159.61	1,619.19	174.36	6.36	219.55	0.46	29.39	0.32	2.15	1,535.4
	OEO	181.45	1,505.23	184.41	6.64	235.63	0.39	29.66	0.33	2.18	1,309.7
A	CON	185.00	1,437.33	177.30	6.50	212.45	0.45	28.75	0.33	2.20	1,577.1
	BS	143.26	1,445.01	165.95	6.45	265.33	0.85	27.65	0.32	2.18	1,647.8
	OEO	151.00	1,364.64	192.93	6.48	215.43	0.55	28.57	0.32	2.20	1,466.5
SEM		13.332	67.948	10.147	0.155	25.804	0.184	0.743	0.007	0.036	175.36
P-values
Egg production		0.3393	0.0069	0.5868	0.7854	0.7670	0.2721	0.0808	0.6136	0.4052	0.2377
Additive		0.1613	0.3181	0.1264	0.5468	0.5837	0.5299	0.7089	0.6593	0.6325	0.5021
Interaction		0.2263	0.9699	0.5318	0.5347	0.4094	0.4876	0.6770	0.4512	0.9915	0.9284

Means in same rows with different superscripts are significantly different (P<0.05).
H, high egg production; A, average egg production; CON, fed a basal diet; BS, fed basal diet plus 3 × 10⁸ CFU/kg feed Bacillus subtilis; OEO, fed basal diet plus 0.3 g/kg feed oregano essential oil.