Table 4. The key amino acid substitutions in VP2 HVR

Strain	Genogroup	Amino acid positions (VP2)
		221	222	242	249	252	253	256	279	284	286	294	299	318	330
A20-CFR-001	G2d (avIBDV)	K	T	V	K	I	Q	V	N	A	I	L	S	D	S
A20-ETC-014	G2d (avIBDV)	K	T	V	K	I	Q	V	N	A	I	L	S	D	S
A20-MRA-149	G2d (avIBDV)	K	T	V	K	I	Q	V	N	A	I	L	S	D	S
19D51	G2d (avIBDV)	K	T	V	K	I	Q	V	N	A	I	L	S	D	S
SHG19	G2d (avIBDV)	K	T	V	K	I	Q	V	N	A	I	L	S	D	S
SHG49	G2d (avIBDV)	K	T	V	K	I	Q	V	N	A	I	L	S	D	S
ZD-2018-1	G2d (avIBDV)	K	T	V	K	I	Q	V	N	A	I	L	S	D	S
Variant E	G2a (avIBDV)	Q	T	V	K	V	Q	V	N	A	I	L	N	D	S
A19-MRA-016	G2a (avIBDV)	K	T	V	K	V	Q	V	N	A	I	L	N	D	S
09D243	G2a (avIBDV)	K	T	V	K	V	Q	V	N	A	I	L	N	D	S
9109	G2b (avIBDV)	Q	T	V	K	V	Q	V	N	A	I	L	N	N	S
19D38	G2b (avIBDV)	Q	T	V	K	V	Q	V	N	A	I	L	N	N	S
F52/70	G1 (cv/at)	Q	P	I	Q	V	Q	V	D	A	T	L	N	G	S
D78	G1 (cv/at)	Q	P	V	Q	V	H	V	N	T	T	L	N	G	R
HK46	G3 (vvIBDV)	Q	A	I	Q	V	Q	I	D	A	T	I	S	G	S
OKYM	G3 (vvIBDV)	Q	A	I	Q	V	Q	I	D	A	T	I	S	G	S
TY-2	G4 (dIBDV)	Q	S	V	Q	V	Q	V	N	A	T	L	H	G	S
A05-HL-144	G4 (dIBDV)	Q	S	V	Q	V	Q	T	N	A	T	L	N	D	S
Mexico04M101	G5	Q	T	V	Q	V	Q	V	N	A	T	L	N	D	S
IBDV/Italy/1829/2011	G6	Q	Q	V	Q	V	E	K	D	A	T	L	S	G	S
V877	G7	Q	P	V	Q	V	Q	V	G	A	T	L	S	G	S