| Target gene | Primer sequence (5’ to 3’) | Tm, °C |
|---|---|---|
| GAPDH | F | 50.0 |
| R | 55.0 | |
| Occludin | F: TGGCCTTCGTCATGCTCAT | 52.6 |
| R: GCTGCACATGGCCAACAAG | 57.9 | |
| JAM2 | F: TGCTCCATGAAGCGAATGC | 52.6 |
| R: TGCCATTACCAGAGCCACAA | 50.0 | |
| ZO-1 | F: TATGAAGATCGTGCGCCTCC | 55.0 |
| R: TGCCGGATAATAGCTGCGTT | 50.0 | |
| Claudin-1 | F: GTGTTTGTTGCTGTGACGGG | 55.0 |
| R: GCCACTCTGTTGCCATACCA | 55.0 |