Table 3. LC-MS/MS condition for vitamin D analysis

Model	AB Sciex Triple Quad 3500
Injection volume	2 μL
Flow rate	0.35 mL/min
Column temperature	40°C
Column	Unison UK-C18(2.0 mm × 100 mm, 3 μm)
Mobile phase	mM Ammonium acetate:methanol(5:95)
Ionization	ESI, positive
Monitoring ion	Vitamin D2: 397 → 107, 105, 91Vitamin D3: 385 → 107, 105, 79
Voltage	5,500
Temperature	500°C