Table 2.
Summary of different methods to estimate in vitro digestibility

(Strain) Reference	Sample wt. (g)	Time (h)				Reagents		Tem.(°C)		pH
		Step 1	Step 2	Step 3	Drying/Ashing	Step 1	Step 2	Step 1	Step 2	Step 1	Step 2
(Pig) Boisen and Fernandez (1995)	1.0	6	18	-	130°C constant wt	25 mL 0.1M phosphate buffer 10 mL 0.2M HCl 1 mL pepsin solution (25 mg/mL) 0.5 mL chloramphenicol	10 mL 0.2M phosphate buffer 5 mL 0.6M NaOH 1 mL porcine pancreatin (50 mg/mL)	39	39	2	6.8
(Pig) Boisen and Fernandez (1997)	0.5	2	4	18	130°C constant wt	25 mL 0.1M phosphate buffer 10 mL 0.2M HCl 1 mL pepsin solution (25 mg/mL) 0.5 mL chloramphenicol	10 mL 0.2M phosphate buffer 5 mL 0.6M NaOH 1 mL porcine pancreatin (50 mg/mL)	39	39	2	6.8
(Pig) Huang et al. (2000)	1.0	4	24			10 mL hydrochloric acid Pepsin solution (1.0 mg/mL HCl)	300 mL of PBS solution 2 mg/mL trypsin solution	37	37	2	7.6
(Pig) Noblet and Jaguelin-Peyraud (2007)	0.5	2	4	-	103°C 4 h /550°C 4 h	25 mL 0.1M phosphate buffer 10 mL 0.2M HCl 1 mL pepsin solution (25 mg/mL) 0.5 mL chloramphenicol	10 mL 0.2M phosphate buffer 5 mL 0.6M NaOH 1 mL porcine pancreatin	39	39	2	6.8
(Pig) Regmi et al. (2009)	1.0	6	18	-		10 mg of pepsin	150 mg pancreatin	39	39	2	6.8
(Pig) Losada et al. (2010)	0.5	2	4	18	80°C constant wt	25 mL 0.1M phosphate buffer 10 mL 0.2M HCl 1 mL pepsin solution (25 mg/mL) 0.5 mL chloramphenicol	10 mL 0.2M phosphate buffer 5 mL 0.6M NaOH 1 mL porcine pancreatin	41	41	2	6.8
(Pig) Chen et al. (2019)	1.0	1.5	3.5			25 mL 0.1M phosphate buffer 2 mL 1 M HCl 1 mL pepsin solution	10 mL 0.2M phosphate buffer 3 mL 1 M NaOH 1 mL pancreatin solution 1 mL bile solution	39	39	3.5	6.8
(Poultry) Valdes and Lesson (1992)	0.5	4	6	-	65°C 48 h	10 mL 0.075 N HCl pepsin solution (20 mg/mL)	4 mL 0.1 N Tris buffer solution 0.1 N NaOH solution 10 mL enzyme solution (40 mg pancreatin, 15 mg bile salts, and 2.5 mg enterokinase)	37	37	4.13	7-7.1
(Poultry) van der klis and Kwakernaak (2014)	0.5	4	6	-	65°C 48 h	10 mL 0.075 N HCl pepsin solution (20 mg/mL)	4 mL 0.1 N tris buffer solution 0.1 N NaOH solution 10 mL enzyme solution (40 mg pancreatin, 15 mg bile salts, and 2.5 mg enterokinase)	37	37	4.13	7-7.1
(Poultry) Losada et al. (2009)	0.5	2	7	-	80°C constant wt	25 mL 0.1M phosphate buffer 10 mL 0.2M HCl 1 mL pepsin solution (25 mg/mL) 0.5 mL chloramphenicol	10 mL 0.2M phosphate buffer 5 mL 0.6M NaOH 1 mL porcine pancreatin (50 mg/mL)	39	39	2	6.8
(Poultry) Yegani et al. (2013)	0.5	2	4	-	65°C overnight	25 mL 0.1M phosphate buffer 10 mL 0.2M HCl (2) 1 mL pepsin solution (25 mg/mL * N) 0.5 mL chloramphenicol	10 mL 0.2M phosphate buffer 5 mL 0.6M NaOH (6.8) 1 mL porcine pancreatin (100 mg/mL * N) 5 mL 20% sulfosalicylic acid	41	41	2	6.8