Table 5. Effects of dietary coenzyme Q10 on the mRNA expression of antioxidant enzymes using qRT-PCR in the liver of laying hens

Item	Treatment1
	CON		PCoQ		ECoQ
	ΔCt2	2−ΔΔCt	ΔCt	2−ΔΔCt	ΔCt	2−ΔΔCt
SOD	4.32±0.54	1	5.44±0.65	0.46	4.68±1.60	0.78
GPX	11.96±1.78	1	13.82±1.51	0.28	13.36±1.27	0.38
CAT	2.98±1.05	1	4.44±0.65	0.36	4.52±1.40	0.34

Treatments are as follows: CON (control diet), PCoQ (control diet supplemented with powdered CoQ10, 100mg/kg diet), and ECoQ (control diet supplemented with emulsified CoQ10, 100mg/kg diet).
The values are ΔCt, which is represented as the Ct of each target gene corrected by Ct of the control gene (RPL27). The fold difference in the relative expression of the target gene was calculated as the 2^−ΔΔCt.