Table 1. Oligo DNA sequence used in this study
ID
Sequence (5’ → 3’)
Usage
c
FMR1
#1 sense-oligo
CACCGGGATCAAGATGCAGTGAAAA
gRNA sequence
c
FMR1
#1 anti-sense-oligo
AAACTTTTCACTGCATCTTGATCCC
gRNA sequence
FMR1
qRT-F
ACAAGAAGAGGGAATGGTACCA
RT-qPCR
FMR1
qRT-R
GCTCCAATCTGTCGAAGCTG
RT-qPCR
FMR1
T7-F
ACCTCTCTATTGTGTCAGCCC
Sequencing
FMR1
T7-R
CACCTCAAACTCCAAGGCCT
Sequencing