Primer | Forward (F) | Reverse (R) | Annealing temperature | |
---|---|---|---|---|
For cloning | CBY2 | 5’-AAA AAG CCA AGC GGA GAC CT-3’ | 5’-AGG CAC TTT CCA GGA AAC AGA-3’ | 56.4℃ |
CBY2-HA | 5’-TTA CAA GCT GTA ATC TGG ACC ATC GTA TGG GTA AAT CTC TCC AAG TTG AAA CTC GTT TG-3’ | - | ||
For quantitative RT-PCR | GAPDH | 5’-GAG GGT AGT GAA GGC TGC TG-3’ | 5’-ACC AGG AAA CAA GCT TGA CG-3’ | 58.0℃ |
CBY2 | 5’-ACC TGA CAA ACC AGA GCA CA-3’ | 5’-CCA GAT GCT CCA CTC GTT CT-3’ | 60.0℃ |