Table 7. The effect of dietary microalgae concentration on the level of cytokines in chicken blood¹,²

Item	Dietary microalgae concentration (%)				SEM	P-value
	0	0.5	1.0	1.5		ANOVA	Linear	Quadratic
IL-1β	73.4	73.2	75.9	78.6	0.91	<0.01	<0.01	0.10
IL-6	55.6	57.1	60.8	61.5	1.14	<0.01	<0.01	0.73

Each value represents the relative protein secretion of mean of eight replicates per treatment.
The value of protein secretion of cytokine is calculated by percentage inhibition (PI) equation; PI = 100 − (Sample O.D value/Max O.D value) × 100.