Table 5. Effects of dietary Zn supplements on the mRNA expression of Zn transporters (ZnT-1 and ZnT-5) and metallothionein (MT) in the liver of Korean native chick

Items	Treatment*						Significance1 (P-value)
	CON		ZnO		ZnM
	ΔCt	2—ΔΔCt	ΔCt	2—ΔΔCt	ΔCt	2—ΔΔCt
ZnT-1	9.73±1.78	1	10.52±3.84	0.58	8.67±2.10	2.09	0.51
ZnT-5	4.78±2.49	1	7.01±2.47	0.21	7.07±1.40	0.21	0.15
MT	3.90±1.07	1	2.59±1.99	2.47	1.85±1.37	4.12	0.10

CON (Control), ZnO (Zinc oxide, 50 ppm) and ZnM (Zn-methionine, 50 ppm).
Significance (P-value) indicated the treatment P-value of Proc GLM procedure.
The values (mean±SD, n=6) are ΔCt, which is represented as the Ct of each target gene corrected by Ct of the control gene (ß-actin).
The fold difference in the relative expression of the target gene was calculated as the 2^—ΔΔCt.