Table 3 The prediction of nsSNPs influence on protein function with polyphen and SIFT in chicken TLR4

#Uploaded-variation	Location	REF	ALT	IMPACT	Feature type	EXON	Protein position	Amino acids	SIFT	SIFT_score	Polyphen	Polyphen 2 score
rs738395510	17:4083135-4083135	A	C	MODERATE	Transcript	1/3	8	T/P	tolerated	0.35	benign	0
rs739102600	17:4083154-4083154	T	G	MODERATE	Transcript	1/3	14	V/G	tolerated	0.21	benign	0.233
rs1058797423	17:4083181-4083181	C	G	MODERATE	Transcript	1/3	23	S/C	deleterious	0.04	probably damaging	0.997
rs1059739146	17:4083190-4083190	C	T	MODERATE	Transcript	1/3	26	A/V	tolerated	0.1	probably damaging	0.991
rs14102161	17:4084296-4084296	A	G	MODERATE	Transcript	2/3	83	K/E	tolerated	1	benign	0
rs732610021	17:4085707-4085707	G	A	MODERATE	Transcript	3/3	225	G/E	deleterious	0.01	probably damaging	0.996
rs312339277	17:4085815-4085815	G	A	MODERATE	Transcript	3/3	261	R/K	tolerated	0.25	benign	0.121
rs15032443	17:4085936-4085936	T	G	MODERATE	Transcript	3/3	301	D/E	tolerated	1	benign	0
rs15032441	17:4086061-4086061	A	G	MODERATE	Transcript	3/3	343	K/R	tolerated	0.41	benign	0.027
rs15032440	17:4086180-4086180	C	T	MODERATE	Transcript	3/3	383	H/Y	tolerated	0.25	benign	0
rs731654157	17:4086779-4086779	G	T	MODERATE	Transcript	3/3	582	L/F	tolerated	0.72	benign	0.003
rs15032438	17:4086865-4086865	G	A	MODERATE	Transcript	3/3	611	R/Q	tolerated	0.43	benign	0
rs735198923	17:4087413-4087413	A	G	MODERATE	Transcript	3/3	794	R/G	deleterious	0	possibly damaging	0.805