Table 2 The prediction of nsSNPs influence on protein function with Polyphen-2 and SIFT in chicken TLR3 (The numbers given in the bracket is amino acid number used from UniProt sequence ID: Q0PQ88)

#Uploaded-variation	Location	REF	ALT	IMPACT	Feature type	EXON	Protein position	Amino acids	SIFT	SIFTscore	Polyphen	Polyphen 2 score
rs80605562	4:61707236-61707236	A	C	MODERATE	Transcript	1/9	12	Q/P	deleterious_low_confidence	0.02	possibly damaging	0.722
rs80692549	4:61707399-61707399	A	T	MODERATE	Transcript	2/9	19(68)	D/V	tolerated	0.47	benign	0.009
rs14479722	4:61707470-61707470	A	C	MODERATE	Transcript	2/9	43(92)	K/Q	tolerated	0.29	benign	0.005
rs14479723	4:61707489-61707489	A	G	MODERATE	Transcript	2/9	49(98)	N/S	tolerated	0.52	benign	0.003
rs731047275	4:61707585-61707585	A	G	MODERATE	Transcript	2/9	81(130)	N/S	tolerated	0.15	benign	0.247
rs80658674	4:61708591-61708591	A	G	MODERATE	Transcript	3/9	131(180)	S/G	tolerated	0.45	benign	0.002
rs80619479	4:61709615-61709615	A	C	MODERATE	Transcript	4/9	174(232)	N/H	tolerated	0.11	benign	0.421
rs80733702	4:61709628-61709628	G	A	MODERATE	Transcript	4/9	178(236)	S/N	tolerated	0.5	benign	0.005
rs80572088	4:61709896-61709896	A	T	MODERATE	Transcript	6/9	201(334)	T/S	tolerated	0.75	benign	0.023
rs80713467	4:61710093-61710093	G	C	MODERATE	Transcript	6/9	266(401)	R/S	tolerated	0.52	benign	0.005
rs80718952	4:61710143-61710143	A	G	MODERATE	Transcript	6/9	283(418)	E/G	tolerated	1	benign	0.001
rs314125162	4:61710434-61710434	G	A	MODERATE	Transcript	6/9	380(515)	R/K	tolerated	1	benign	0
rs794518640	4:61710634-61710634	A	G	MODERATE	Transcript	6/9	447(582)	K/E	tolerated	0.16	possibly damaging	0.63
rs80778047	4:61710677-61710677	C	T	MODERATE	Transcript	6/9	461(596)	A/V	tolerated	0.36	benign	0.043
rs80668052	4:61710691-61710691	G	C	MODERATE	Transcript	6/9	466(601)	D/H	tolerated	0.13	benign	0.051
rs80636705	4:61710832-61710832	G	T	MODERATE	Transcript	6/9	513(648)	A/S	tolerated	0.24	benign	0.008
rs80641674	4:61711004-61711004	C	T	MODERATE	Transcript	6/9	570(705)	A/V	tolerated	0.41	benign	0.002
rs80747649	4:61711195-61711195	A	T	MODERATE	Transcript	6/9	634(769)	T/S	tolerated	0.27	benign	0.001
rs80669462	4:61711252-61711252	G	A	MODERATE	Transcript	6/9	653(788)	E/K	tolerated	0.2	possibly damaging	0.894
rs80661166	4:61711293-61711293	A	G	MODERATE	Transcript	6/9	666(801)	I/M	tolerated	0.23	probably damaging	0.995
rs80757506	4:61712065-61712065	G	A	MODERATE	Transcript	7/9	716(851)	D/N	tolerated	0.09	possibly damaging	0.784
rs735041008	4:61715833-61715833	C	T	MODERATE	Transcript	8/9	763	P/L	deleterious_low_confidence	0.04	unknown	?
rs740030255	4:61715851-61715851	T	G	MODERATE	Transcript	8/9	769	V/G	deleterious_low_confidence	0	unknown	?
rs316043187	4:61715914-61715914	A	G	MODERATE	Transcript	8/9	790	N/S	deleterious_low_confidence	0	unknown	?
rs736982671	4:61715944-61715944	A	G	MODERATE	Transcript	8/9	800	Q/R	deleterious_low_confidence	0	unknown	?
rs733651632	4:61715958-61715958	T	G	MODERATE	Transcript	8/9	805	S/A	deleterious_low_confidence	0	unknown	?
rs316084677	4:61716029-61716029	G	T	MODERATE	Transcript	8/9	828	R/S	deleterious_low_confidence	0	unknown	?
rs14479729	4:61720359-61720359	C	T	MODERATE	Transcript	9/9	830	P/L	deleterious_low_confidence	0	unknown	?
rs14479730	4:61720422-61720422	C	T	MODERATE	Transcript	9/9	851	A/V	deleterious_low_confidence	0	unknown	?
rs14479732	4:61720444-61720444	T	A	MODERATE	Transcript	9/9	858	H/Q	deleterious_low_confidence	0	unknown	?
rs793896212	4:61720447-61720447	A	C	MODERATE	Transcript	9/9	859	Q/H	deleterious_low_confidence	0	unknown	?
rs318110846	4:61720512-61720512	A	G	MODERATE	Transcript	9/9	881	D/G	deleterious_low_confidence	0	unknown	?
rs1057909073	4:61720562-61720562	C	T	MODERATE	Transcript	9/9	898	P/S	deleterious_low_confidence	0	unknown	?
rs1057733301	4:61720567-61720567	C	G	MODERATE	Transcript	9/9	899	C/W	deleterious_low_confidence	0	unknown	?
rs738876733	4:61720632-61720632	G	A	MODERATE	Transcript	9/9	921	G/D	deleterious_low_confidence	0	unknown	?