Table 5 Effect of dietary Lactobacillus, medicinal plants extract, herb extract on the leucocyte profiles in laying hens against Salmonella gallinarum (n=10)

Challenge White blood cell (K/μL) Heterophil/Lymphocyte ratio
4d 8d 14d Total 4d 8d 14d Total
Treatments NC × 17.04 13.93 10.43 13.80 0.33 0.16 0.13 0.21
27.96 18.00 11.23 19.06 0.94 0.28 0.16 0.46
PC × 15.65 19.77 10.06 15.16 0.40 0.43 0.17 0.33
29.01 22.77 6.84 19.54 0.73 0.41 0.13 0.42
LB × 17.88 11.61 16.76 15.42 0.46 0.29 0.35 0.37
28.26 18.06 19.25 21.86 0.72 0.35 0.30 0.46
MPE × 20.92 13.03 12.51 15.49 0.45 0.24 0.19 0.29
29.79 19.18 15.62 21.53 0.77 0.48 0.25 0.50
EO × 14.35 17.48 12.09 14.64 0.30 0.30 0.13 0.24
27.94 20.50 15.72 21.39 0.81 0.45 0.29 0.52
SEM 1.98 1.12 1.18 1.01 0.07 0.03 0.03 0.03
Challenge without Salmonella 17.17 15.16 12.37 14.90 0.39 0.28 0.19 0.29
with Salmonella 28.59 19.70 13.73 20.68 0.79 0.39 0.23 0.47
Feed NC 22.50 15.97 10.83 16.43 0.64 0.22 0.15 0.33
PC 22.33 21.27 8.45 17.35 0.57 0.42 0.15 0.38
LB 23.07 14.84 18.01 18.64 0.59 0.32 0.33 0.41
MPE 25.36 16.11 14.07 18.51 0.61 0.36 0.22 0.40
EO 21.15 18.99 13.91 18.01 0.56 0.38 0.21 0.38
Challenge NS NS NS NS NS NS NS NS
Feed NS NS NS NS NS NS NS NS
Challenge × feed NS NS NS NS NS NS NS NS
NC, basal diet; PC, basal diet with virginiamycin 10 ppm + salinomycin 60 ppm; LB, Lactobacillus 0.1%; MPE, medicinal plants extract 100 ppm; EO, essential oil 100 ppm.NS: none significant.