Table 5 Effect of dietary Lactobacillus, medicinal plants extract, herb extract on the leucocyte profiles in laying hens against Salmonella gallinarum (n=10)
| Challenge | White blood cell (K/μL) | | Heterophil/Lymphocyte ratio |
4d | 8d | 14d | Total | | 4d | 8d | 14d | Total |
Treatments | NC | × | 17.04 | 13.93 | 10.43 | 13.80 | | 0.33 | 0.16 | 0.13 | 0.21 |
○ | 27.96 | 18.00 | 11.23 | 19.06 | | 0.94 | 0.28 | 0.16 | 0.46 |
PC | × | 15.65 | 19.77 | 10.06 | 15.16 | | 0.40 | 0.43 | 0.17 | 0.33 |
○ | 29.01 | 22.77 | 6.84 | 19.54 | | 0.73 | 0.41 | 0.13 | 0.42 |
LB | × | 17.88 | 11.61 | 16.76 | 15.42 | | 0.46 | 0.29 | 0.35 | 0.37 |
○ | 28.26 | 18.06 | 19.25 | 21.86 | | 0.72 | 0.35 | 0.30 | 0.46 |
MPE | × | 20.92 | 13.03 | 12.51 | 15.49 | | 0.45 | 0.24 | 0.19 | 0.29 |
○ | 29.79 | 19.18 | 15.62 | 21.53 | | 0.77 | 0.48 | 0.25 | 0.50 |
EO | × | 14.35 | 17.48 | 12.09 | 14.64 | | 0.30 | 0.30 | 0.13 | 0.24 |
○ | 27.94 | 20.50 | 15.72 | 21.39 | | 0.81 | 0.45 | 0.29 | 0.52 |
SEM | | 1.98 | 1.12 | 1.18 | 1.01 | | 0.07 | 0.03 | 0.03 | 0.03 |
Challenge | without Salmonella | | 17.17 | 15.16 | 12.37 | 14.90 | | 0.39 | 0.28 | 0.19 | 0.29 |
with Salmonella | | 28.59 | 19.70 | 13.73 | 20.68 | | 0.79 | 0.39 | 0.23 | 0.47 |
Feed | NC | | 22.50 | 15.97 | 10.83 | 16.43 | | 0.64 | 0.22 | 0.15 | 0.33 |
PC | | 22.33 | 21.27 | 8.45 | 17.35 | | 0.57 | 0.42 | 0.15 | 0.38 |
LB | | 23.07 | 14.84 | 18.01 | 18.64 | | 0.59 | 0.32 | 0.33 | 0.41 |
MPE | | 25.36 | 16.11 | 14.07 | 18.51 | | 0.61 | 0.36 | 0.22 | 0.40 |
EO | | 21.15 | 18.99 | 13.91 | 18.01 | | 0.56 | 0.38 | 0.21 | 0.38 |
Challenge | | NS | NS | NS | NS | | NS | NS | NS | NS |
Feed | | NS | NS | NS | NS | | NS | NS | NS | NS |
Challenge × feed | | NS | NS | NS | NS | | NS | NS | NS | NS |
NC, basal diet; PC, basal diet with virginiamycin 10 ppm + salinomycin 60 ppm; LB, Lactobacillus 0.1%; MPE, medicinal plants extract 100 ppm; EO, essential oil 100 ppm.NS: none significant.