Table 2 Analysis method of the fatty acid composition using gas chromatography

Item Condition
Instrument Varian cp 3800. U.S.A
Column Omegawax 320 (30 m × 0.32 μm × 0.25 mm)
Injection temperature 250℃
Detector temperature 260℃
Detector Flame ionization detector(FID)
Oven temperature 200℃ for 45 min
Carrier gas N2 at 1.0 mL/min constant flow
Split ratio 100:1
Gas flow rate 1.0 mL/min
Injection volume 1.5 μL